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HPLC HINTS & TIPS for Chromatographers

From time to time we will add various 'hints and tips' to this web page which we feel are of interest to anyone performing HPLC, UHPLC, LC-MS  or SFC analysis. Many of these tips are taught in our specialized training courses. Often, they are the result of problems we see people experiencing in the field. As scientific educators and consultants, we try to pass on any practical information that may help others improve the way in which they conduct their research.

We welcome your comments and suggestions on these so please feel free to let us know if you find any of these helpful in your work or if you have a suggestion for one.


         TABLE OF CONTENTS:

 

TIP #

DESCRIPTION AND WEB PAGE LINK (click to view)

DATE ADDED

101

Inline HPLC Degassing Systems / Modules

05/29/2010

102 Reference Wavelengths (as used in HPLC UV/VIS Detectors)

05/30/2010

103 Flow Cell Volume & Path Length (Method Transfer Issues)

06/12/2010

104 Chiral HPLC / SFC Method Development (Alcohol's)

07/07/2010

105 Power & Surge Protection for Computers & Analytical Instruments

07/18/2010

106 Determination of HPLC Column Dead Time / Dwell Volume, (T0)

08/01/2010

107 Chiral HPLC and SFC Column Screening Strategies

09/20/2010

108 HPLC TO UHPLC Conversion Notes

11/22/2010

109 UV VIS Detector Signal Bandwidth

12/06/2010

110 HPLC Pump Solvent Compressibility Values

01/16/2011

111 Proteomics and LC-MS, MS/MS Software Tools

04/21/2011

112 Determining the Data Acquisition Rate For Your HPLC Detector

05/30/2011

113 How Do C18 Phases Differ?

07/18/2011

114 Calculate Pressure Drop Across an HPLC / UHPLC Column

08/17/2011

115 Adduct Formation In LC-MS Analysis (Esp. ESI)

11/17/2011

116 Hydrophilic Interaction Chromatography (HILIC)

02/21/2012

117 Techniques To Enhance Negative Mode ESI in LC/MS

04/17/2012

118 Optional Seal Wash & Flushing the HPLC System

08/24/2012

119 Common HPLC UHPLC Calculations

01/01/2013

120 Adjusting Mobile Phase Flow Rate to Maintain HPLC / UHPLC Linear Velocity When Column Diameter Is Changed

04/01/2013

 


Chiralizer™ Services, L.L.C.  USA

Copyright 2010 - 2013, Chiralizer™ Services, L.L.C. All Rights Reserved. Chiralizer, Column Swapper & LC Spiderling are trademarks of Chiralizer Services, LLC  USA. *These "Hints & Tips" are protected by copyright and are not to be copied or duplicated in whole or part without the written permission of Chiralizer Services, LLC.